Vespidae_Herz_06

Sample information

Picture
Photos by: Lea R., Louisa R.
Location
Collection date 08/25/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

captured: in the garden on a pear in Oberweningen

condition: dead when caught, then frozen and preserved in alcohol

Putative identification Arthropoda Hexapoda Insecta Hymenoptera Vespidae

Methods

Extraction kit Instagene Matrix
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

In both PCR processes meaningful/good results could be observed. We refer to the results in lane 8 (picture 1) and lane 3 (picture 2).

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

In both PCR processes meaningful/good results could be observed. We refer to the results in lane 6 (picture 1) and lane 2 (picture 2). In the first process you can see a soft/weak line/mark on the gel. It’s not as strong as in the other two lanes that showed a mark.

 

Sequence quality: very poor/ failed
We took a short (41 bases) section of the DNA and there must have been caught a CO gene, which is the same in many different species, only if we had a larger section of the DNA we would have seen differences. We would have to do the sequencing again.
The results showed the sequence matched with “Chalarus Decorus”, “Pipunculidae” and “Eriocrania”.
“Chalarus Decorus” is a species of fly in the family Pipunculidae. The insect we caught was a wasp, so it must have been a common gene segment from common ancestors.

Chalarus Decorus:

 

source of the picture:
https://commons.wikimedia.org/wiki/File:Verrallia_aucta.jpg
Wikipedia:
https://en.wikipedia.org/wiki/Chalarus_decorus

Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Vespidae was found to be negative for Wolbachia.
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