Sample information |
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Picture |
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Location | |
Collection date | 08/25/2024 |
Captive / Cultivated? | Wild-caught |
Group | Kantonsschule Zuercher Unterland |
Observations | captured: in the garden on a pear in Oberweningen condition: dead when caught, then frozen and preserved in alcohol |
Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Vespidae |
Methods |
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Extraction kit | Instagene Matrix |
DNA extraction location | |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniOne |
Buffer | TBE |
DNA stain | Midori 1:20'000 |
Gel images |
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Protocol notes | In both PCR processes meaningful/good results could be observed. We refer to the results in lane 8 (picture 1) and lane 3 (picture 2). |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | In both PCR processes meaningful/good results could be observed. We refer to the results in lane 6 (picture 1) and lane 2 (picture 2). In the first process you can see a soft/weak line/mark on the gel. It’s not as strong as in the other two lanes that showed a mark.
Sequence quality: very poor/ failed Chalarus Decorus:
source of the picture: |
Wolbachia 16S sequence | |
Arthropod COI sequence | Download FASTA
Download AB1
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Summary | The Vespidae was found to be negative for Wolbachia. |