Millipede_AG wolbochia_lab_hendricks

March 16, 2026

Sample information
Picture
Entry by: Abhijeet G
Location
Collection date 11/06/2025
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Light brown shell, whitish underside, under 1.5cm long, slow to moderate walking pace, paired legs, 2 antennae

Found under pine straw in a natural pine forest
Putative identification Arthropoda Diplopoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

DNA was sequenced, but low CRL led to non-specific results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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