Episyrphus balteatus_Kreis_4

October 24, 2024

Sample information
Picture
Photos by: Anja H.
Location
Collection date 08/25/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

In gras next to stream.
Putative identification Arthropoda Insecta Diptera Syrphidae Episyrphus Episyrphus balteatus

Methods
Extraction kit Instagene Matrix
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

Reasons why it didn‘t work during the first try (Picture 1):

– Foam formation (broken machine)

– to much/few sample pipetted in the gel

– Centrifuged for too little time

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Episyrphus balteatus (Hainschwebfliege/ Grove Hoverfly)

https://de.wikipedia.org/wiki/Hainschwebfliege

DNA tested.

 
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence Download FASTA    Download AB1
Summary The Episyrphus balteatus was found to be postive for Wolbachia.
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