Formica cunicularia_ZuercherUnterland_smiley

October 24, 2024

Sample information
Picture
Photos by: Julia C., Svenia H.
Location
Collection date 08/18/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

Caught alive in grass, frozen to death and stored in alcohol until DNA-extraction
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Formica

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

ArthropodPCR-Gel2: During this PCR round arthropodic DNA was targeted. The relevant sample is located in lane 7.

WolbachiaPCR-Gel9: Here wolbachia was targeted. The relevant sample is located in lane 8.

The  results show that the sample was infected with wolbachia

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The band is clearly visible.
There is a band that indicates wolbachia presence. This can be trusted because other samples on the same gel tested positive for wolbachia too. The control sample is clearly recognizable as well, which means the PCR-process was successful. Unfortunatly, the sample likely got damaged during transport to the US. The sequence was unusable and therefore we could not prove the presence of Wolbachia through the use of the BLAST-tool.

(Additionally, since the first round didn‘t generate usable results, the PCR was repeated by the biology teacher, which makes the results more credible.)
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence Download FASTA    Download AB1
Summary The Formica was found to be postive for Wolbachia.
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