Sample information |
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Picture |
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Location | |
Collection date | |
Captive / Cultivated? | Wild-caught |
Group | Georgia Southern University |
Observations |
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Putative identification | Arthropoda Hexapoda Insecta Lepidoptera |
Methods |
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Extraction kit | |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | Other |
Gel images |
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Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | My confidence level is medium because I tested the DNA in the gel electrophoresis, and it tested positive for Wolbachia, but it could also be an error, and my sequence did not turn out for Wolbachia. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
>01D_GREAA-MT_CO1F TRIM QUALITY: 20 TACTTCTTTAAGTTTATTAATTCGAACAGAATTagccatcccAGGATCTT TAATTGGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTT ATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAA TTGATTAGTTCCTTTAATATTAGGGGCTCCTGATATAGCTTTTCCTCGTA TAAATAATATAAGATTTTGGATACTTCCTCCTTCTCTAACTTTATTAATT TCAAGAAGTATTGTAGAAAATGGAGCAGGAACAGGTTGAACAGTTTATCC TCCTCTTTCTTCTAATATTGCTCATCAAGGAGCTTCTGTTGATTTAGCAA TTTTTTCTCTTCATTTAGCAGGTATTTCATCTATTTTAGGAGCTATTAAT TTTATTACTACAATTATTAATATACGAATTAAAAATTTATCATTTGATCA AATACCTTTATTTGTTTGATCTGTTGGTATTACAGCCTTATTATTACTTT TATCTTTACCAGTTTTAGCAGGAGCTATTACTATACTTTTAACTGATCGA AATTTAAATACTTCATTTTTTGATCCTGCTGGAGGAGGAGATCCAATTTT ATATCAACATTTATTTTGATTTTTTGGTCACC
BLAST at The Wolbachia Project BLAST at NCBI
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Summary | The Lepidoptera was found to be negative for Wolbachia. |