MATAEF24-JLR Hemiptera

November 5, 2024

Sample information
Picture
Photos by: Jamie R.
Location
Collection date 08/27/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Around 10 am, humid and warm outside, in the long grass behind the science center building, used sweep method to catch
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident that my insect is of the order Hemiptera based off of the insect CO1 gene and my research done on it. I am also confident that my insect is not infected with Wolbachia since my positive and negative controls results were as expected, which ensures my trust in my results.
Wolbachia 16S sequence
Arthropod COI sequence
TAtgAGCAGGTATAGTAGGTTCCGCCATAAGAATCATCATTCGTATTGAA CTAGGACAACCAGGAAGATTTAttggggGATGACCAAATCTATAATGTAG TAGTCACCGCTCACGCATTCGTAATAATTTTCTTTATAGTAATACCAATT ATAATTGGGGGTTTTGGTAACTGATTAGTACCATTAATAATTGGAGCCCC TGATATAGCATTTCCTCGTATAAATAATATAAGATTCTGACTATTACCCC CTTCAATTACATTATTAATAATAAGTAGATTGGCTGAATCAGGAGCAGGG ACTGGTTGAACCGTCTACCCCCCATTATCTAGTAATCTATCACACAGAGG AGCATCAGTAGACTTAGCTATTTTTTCTCTACATCTAGCAGGAGTATCCT CAATCCTAGGAGCTGTAAATTTCATCTCAACAATCATTAATATACGCCCA GAAGGAATAATTCCAGAACGAATTCCCTTATTTGTTTGATCAGTAGGGAT CACCGCTCTTCTTCTTCTTCTATCACTTCCTGTATTAGCAGGAGCTATCA CCATACTATTAACTGACCGAAATTTTAATACTTCCTTCTTTGACCCTTCA GGAGGGGGAGACCCTATTTTATATCAACATTTATTTTGATTTTTTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
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