DEMAHF24-VLB Diptera

November 5, 2024

Sample information
Picture
Photos by: Vanessa L.
Location
Collection date 08/27/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Environment: Dry grass in a very green area with lots of fields and bushes

Temperature: Hot and humid

Time: 1:05 in the afternoon
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My confidence level in the accuracy of my experiment is high because there was many steps and procedures we followed that prevented any mistakes during the process of the PCR.
Wolbachia 16S sequence
Arthropod COI sequence
AGTATATTAATTCGAACAGAATTAAgccaTCCAGGATTTTTTATTGGTGA TGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTATTATAATTT TTTTTATAGTTATACCAATTATAATTGGTGGATTTGGAAACTGATTAGTT CCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGAATAAATAATAT AAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCA TAGTTGAAAACGGGGCTGGTACTGGATGAACAGTATATCCCCCTCTTTCT AGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTTTTTCTCT TCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTA CAGTTATTAATATGCGATCTACTGGAATTACATTTGATCGTATACCACTT TTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCC AGTTTTAGCCGGGGCTATTACTATACTATTAACAGATCGAAATTTAAATA CAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCTATTTTATACCAACAT TTATTTTGATTTTTTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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