DEMAHF24-ZDG, Diptera

November 5, 2024

Sample information
Picture
Photos by: Zaniya G.
Location No map location data available.
Collection date 08/27/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Environment: Dry Grassy Area, Very Warm Climate
  • Location: West of Jenkins Hall on Georgia Southern University – Armstrong Campus
  • Time of Collection: 1:05 PM
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I’m very confident in my experiment because the controls came out as they were supposed to as pertaining to the number of bands they were supposed to have and they contained the specific bands that they were supposed to have. For example, the positive control had both the insect and Wolbachia band, the negative control had just the insect band, and the NTC had no bands at all.
Wolbachia 16S sequence
Arthropod COI sequence
GAACTTCACTAAGTATATTAATTCGAACAGAATTaacccttccAGGATTT TTTATTGGTGATGATCAAATTTATAATGtcaTTGTTACAGCCCATGCTTT TATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTTGGAA ACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGA ATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCT AATAAGAAGCATAGTTGAAAACGGGGCTGGTACTGGATGAACAGTATATC CCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCA ATTTTTTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAA TTTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACATTTGATC GTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTC CTATCCCTCCCAGTTTTAGCCGGGGCTATTACTATACTATTAACAGATCG AAATTTAAATACAAGTTTCTTTGACCCAGCaggaggggGAGACCCTATTT TATACCAACATTTATTTTGATTTTTTGGTCACCCTGAAGTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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