Sample information |
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Picture |
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Location | |
Collection date | 08/27/2024 |
Captive / Cultivated? | Wild-caught |
Group | Georgia Southern University |
Observations |
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Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
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Extraction kit | |
DNA extraction location | Partial abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | Other |
Gel images |
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Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
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Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | I had predicted my sample would be positive forĀ Wolbachia, but it was not. I was confident in my skills to carry out the procedure correctly, but my unsupportive results dampened my confidence a bit. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
AGGAACTTCCTTAAGATGACTTATCCGAATCGAACtacgcctgcCAGGAT CATTCATTGGTGATGATCAAATTTATAATGTAGTAGTAACTGCCCATGCA TTCATTATAATCTTTTTTATAGTGATGCCAATTATGATCGGAGGATTTGG TAACTGACTAGTTCCTTTAATACTGGGGGCCCCAGATATAGCATTCCCAC GAATAAATAACATAAGATTCTGATTACTGCCCCCCTCACTAACTCTTCTT TTATCAAGTAGAATCGCCGAAAGAGGGGCCGGAACTGGATGAACAGTATA TCCTCCCCTATCTACTAATATTTCACACAGTGGAGCATCCGTAGATTTAG CTATTTTTTCATTACATTTAGCTGGTGTTTCCTCAATTTTAGGTGCTGTT AATTTTATTTCAACAATTATTAACATGCGAGCCACAGGAATAACCTCCGA CCGAATACCTTTATTTGTATGATCCGTAGGTATTACCGCCCTTCTTTTAC TTTTATCACTACCTGTACTTGCCGGAGCAATTACTATACTATTAACAGAT CGAAATTTAAATACCTCATTTTTCGATCCAGCAGGAGGAGGGGACCCAAT TCTTTATCAACACTTGTTTTGATTTTTTG
BLAST at The Wolbachia Project BLAST at NCBI
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Summary | The Hemiptera was found to be negative for Wolbachia. |