WRIAGF24-06 HYMENOPTERA

November 6, 2024

Sample information
Picture
Location
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit none
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I correctly extracted DNA and my prediction was supported.
Wolbachia 16S sequence
Arthropod COI sequence
ATTAGGTTCATCTATAAGATTAATTATTCGTATAGAACTAGGTATAGCAG GTTCATTACTTAAAAATGAACAATTATTTAATTCAATCGTTACTTCACAT GCATTCGTAATAATTTTCTTTTTTATTATGCCTCTAATAATTGGAGGTTT TGGCAATTGATTAATTCCTCTAATATTGGGAGTACCTGATATAGCTTTCC CTCGATTAAATAATTTAAGATTTTGACTATTACCTCCCTCTCTTATTTTA TTAATTTTTTCTAACTTTGTAGGAAAAGGAATTGGGACAGGATGAACTAT TTATCCCCCACTATCTAATTCTATTTACCATACTGGTATAGCAGTAGATT TATTAATTTATTCCCTACATTTAAGAGGAATTTCTTCAATTCTAGGCTCA ATTAATTTTATTGTAACAATTTTTAAAATACATAATTCTAATATTAAAAT ACATTTAGTAATATTATTTCCTTGATCTATTTATATTACTACAATTTTAT TATTACTTTCATTACCAGTTTTAGCTGGGGCAATTACTATACTTTTATTT GATCGAAACTTTAATACTTCATTTTTTGATCCCTCAGGAGGAGGAGATCC AGTTTTATATCAACATTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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