WRIAGF24-08 Coleoptera

November 6, 2024

Sample information
Picture
Photos by: Ava H
Location No map location data available.
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found on sunny day by a tree at Georgia Southern Armstrong Campus, Science Center.

It was 2pm and 89 degrees outside.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident in my results because my controls worked and I followed all protocols.
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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