WRIAGF24-13 Hymenoptera

November 6, 2024

Sample information
Picture
Photos by: Ryley L
Location No map location data available.
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found in bushes outside Lane Library in GSU. Used “catch and flip” method with the sweep net.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls all worked correctly and the Wolbachia band was not present.
Wolbachia 16S sequence
Arthropod COI sequence
TGCAGCTATAAGAATAATTATTCGATTAGAACTAGGAACACCTGGAAAAT TCATTGGTAATGATCAAATTTATAATACTTTAGTTACTGCTCACGCATTT ATCATAATTTTTTTCATAGTTATACCATTTATAATTGGAGGATTTGGAAA TTGATTAATTCCATTAATATTAACAGCTCCTGATATAGCCTTTCCCCGTA TAAATAATATAAGATTTTGATTACTACCTCCTTCATTAATTTTTTTATTA TCAAGTAGTATTATTGGTTCAGGTGTAGGAACAGGTTGAACCCTTTACCC TCCTTTATCATCTATTATTGGACATAATTCTCCATCAGTAGATTTAAGAA TTTTTGCTTTACATATTGCTGGAGTATCATCTATTATAGGAGCTATTAAT TTTATTGTAACAATTTTAAATATACATACTAAAAATTCTTCCTTAAATTC AATCCCACTTTTTGCTTGATCTGTTCTTATTACTGCAATTCTATTACTTC TATCTTTACCAGTATTAGCTGGAGCCATCACCATATTATTAACTGATCGA AATTTTAATACATCATTTTTTGACCCTGCTGGAGGAGGTGATCCAATCCT TTATCAACATTTATTTTGATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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