Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 08/28/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Found in bushes outside Lane Library in GSU. Used “catch and flip” method with the sweep net. |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The controls all worked correctly and the Wolbachia band was not present. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TGCAGCTATAAGAATAATTATTCGATTAGAACTAGGAACACCTGGAAAAT TCATTGGTAATGATCAAATTTATAATACTTTAGTTACTGCTCACGCATTT ATCATAATTTTTTTCATAGTTATACCATTTATAATTGGAGGATTTGGAAA TTGATTAATTCCATTAATATTAACAGCTCCTGATATAGCCTTTCCCCGTA TAAATAATATAAGATTTTGATTACTACCTCCTTCATTAATTTTTTTATTA TCAAGTAGTATTATTGGTTCAGGTGTAGGAACAGGTTGAACCCTTTACCC TCCTTTATCATCTATTATTGGACATAATTCTCCATCAGTAGATTTAAGAA TTTTTGCTTTACATATTGCTGGAGTATCATCTATTATAGGAGCTATTAAT TTTATTGTAACAATTTTAAATATACATACTAAAAATTCTTCCTTAAATTC AATCCCACTTTTTGCTTGATCTGTTCTTATTACTGCAATTCTATTACTTC TATCTTTACCAGTATTAGCTGGAGCCATCACCATATTATTAACTGATCGA AATTTTAATACATCATTTTTTGACCCTGCTGGAGGAGGTGATCCAATCCT TTATCAACATTTATTTTGATTTTTT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid