Sample information |
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Picture |
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Location | |
Collection date | 08/28/2024 |
Captive / Cultivated? | Wild-caught |
Group | Georgia Southern University |
Observations | Caught outside school on a warm day, caught by hand after finding on the concrete. |
Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
Extraction kit | |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | Other |
Gel images |
|
Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | I was not expecting Wolbachia and results did not yield a Wolbachia presence. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Hymenoptera was found to be negative for Wolbachia. |