Sample information |
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Picture |
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Location | |
Collection date | 08/17/2020 |
Captive / Cultivated? | Wild-caught |
Group | St. John High School USD 350 |
Observations | The box elder bugs were found on a tree and seemed to be apart of a colony. The bugs estimated infection rate is 12.5%. |
Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
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Extraction kit | DNeasy (Qiagen) |
DNA extraction location | Abdomen |
Single or Duplex PCR | |
Gel electrophoresis system | MiniPCR |
Buffer | TBE |
DNA stain | GelGreen |
Gel images |
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Protocol notes | The boxelder bugs were stored in 90% ethanol for about six months prior to extraction. DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete. PCR: Mini One Taq polymerase was used. Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear. |
Results |
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Wolbachia presence | Yes |
Confidence level | High |
Explanation of confidence level | Lane 7 was double banded and the Wolbachia band matched up with the positive control. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Hemiptera was found to be postive for Wolbachia. |