Ants Period 3 remote group 3

February 2, 2021

Sample information
Picture
Photos by: Nathan W.
Location
Collection date 10/01/2020
Captive / Cultivated? Wild-caught
Group Acton-Boxborough Regional High School
Observations

This was found in a pitfall trap baited with honey at the edge of the woods.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The control match wasn’t good, but it did work a little bit, which validates these results.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
NNNNNNNNNNNNNNGNNNNNNNNNTNCTNNNNNTNNATCCTGNAANTCCNGAGNNNNGANTGGNNNNCTACCNTATNNNNNNNNNNNNNNNNNNNNNNTTANN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formicidae was found to be negative for Wolbachia.
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