Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 10/01/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Acton-Boxborough Regional High School |
| Observations | This was found in a pitfall trap baited with honey at the edge of the woods. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The control match wasn’t good, but it did work a little bit, which validates these results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
NNNNNNNNNNNNNNGNNNNNNNNNTNCTNNNNNTNNATCCTGNAANTCCNGAGNNNNGANTGGNNNNCTACCNTATNNNNNNNNNNNNNNNNNNNNNNTTANN
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Formicidae was found to be negative for Wolbachia. |
Water Boatman