Wolbachia Project #3 grasshopper

Sample information
Picture	Photos by: Bineta K
Location
Collection date	11/01/2024
Captive / Cultivated?	Wild-caught
Group	Davies Career and Technical High School
Observations	It was hopping in a feild near a different building. It was caught while resting
Putative identification	Arthropoda
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Partial abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	The bug doesn’t have Wolbachia but it showed up in the control. My arthropod had no band which means the grasshopper did not have Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Arthropoda was found to be negative for Wolbachia.

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