Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/01/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Davies Career and Technical High School |
| Observations | Running fast trying to hide under leaves, near a fallen tree. |
| Putative identification | Arthropoda Chelicerata Arachnida |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I’m confident because my Wolbachia lane for my Wolf Spider came out blank, but the fruit flies appeared, so the Taq Mater Mix worked my bug didn’t have any Wolbachia at all. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arachnida was found to be negative for Wolbachia. |
Water Boatman