Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | The larvae was burrowed into a piece of dead wood. |
| Putative identification | Arthropoda Insecta Coleoptera Cerambycidae |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Larva |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | My sample is in lane 5. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | On the Wolbachia gel there was no bar at 438 base pairs for lane 5, indicating that there was no Wolbachia DNA present in my sample. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
NNNNNNNNNNATTTTTGGAGCNTGAGCAGGNATANTTGGAACTTCTCTAAGACTCCTAATTCGTACAGAACTAGGAAACCCTGGATCTTTAATTGGTGACGATCAAATTTATAATGTTATTGTTACCGCTCATGCATTTATTATAATTTTTTTTATAGTTATACCTATTATAATCGGGGGGTTTGGAAATTGATTAGTTCCCTTAATATTAGGAGCACCAGATATAGCTTTCCCTCGTATAAATAATATAAGATTTTGATTACTTCCTCCCTCATTAACATTATTAATTATAAGAAGAATTGTAGAAAGAGGGGCTGGTACAGGATGAACTGTATACCCACCCCTATCTTCTAACATTGCTCACGGAGGTTCTTCTGTTGATTTGGCTATTTTCAGTCTTCATTTAGCAGGTATTTCTTCCATTTTAGGGGCAGTTAACTTTATCACTACAGTTATTAATATACGACCAGTTGGAATAAGACCAGATCGAATACCTCTCTTTGTTTGAGCTGTTGTAATTACTGCTGTTCTTTTATTACTCTCTTTACCTGTCTTAGCAGGGGCAATTACTATACTTTTAACAGATCGTAATCTTAATACTTCATTTTTTGATCCAGCTGGTGGGGGAGACCCAATTTTATACCAACATCTATTTTGATTTTTTGGTCNCNNN
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Cerambycidae was found to be negative for Wolbachia. |
My Data
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data