Joro Spider

November 20, 2024

Sample information
Picture
Photos by: Emily B
Location
Collection date 11/12/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Found on a web at the top of a tree. Tree in a parking lot.
Putative identification Arthropoda Chelicerata Arachnida Araneae Nephilidae Trichonephila Trichonephila clavata

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

The imaging results came back below 40 and, therefore, were of poor quality. Above 40 is quality results but we can not be sure, even though the BLAST came back positive.
Wolbachia 16S sequence
Arthropod COI sequence
>7_Emily_spider-CO1F ACTGCAATAAGAGTATTAATTCGTACTGAGCTTGGACAGCCAGGAAGATTTATAGGGGATGATCAATTATATAATGTAAT TGTTACAGCTCATGCATTTGTGATAATTTTTTTTATAGTAATACCAATTTTAATTGGTGGATTTGGAAATTGATTGGTTC CTTTAATATTAGGAGCGCCAGATATAGCGTTTCCTCGAATAAATAATTTAAGGTTTTGACTATTGCCTCCTTCTTTATTT TTATTGTTCGTTTCTTCAATAGTAGAGATAGGAGTGGGAGCAGGTTGGACAGTTTACCCTCCTTTATCATCTTTAGATGG ACATTCTGGAAGGTCAGTGGATTTTGCTATTTTTTCTTTACATTTAGCTGGGGCTTCTTCAATTATAGGGGCTATTAATT TCATTTCTACTATTGTGAATATACGATCTTTTGGAATAACTATAGAAAAGGTACCTTTATTTGTTTGGTCTGTTTTAATT ACTGCTGTTTTATTACTTTTATCTTTACCTGTATTAGCAGGTGCTATTACAATATTATTGACTGATCGAAATTTTAATAC ATCATTTTTTGACCCTTCTGGAGGGGGGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Trichonephila clavata was found to be postive for Wolbachia.
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