DKH 6

November 21, 2024

Sample information
Picture
Location
Collection date 08/29/2024
Captive / Cultivated? Wild-caught
Group Auburn University at Montgomery
Observations
Putative identification Arthropoda Hexapoda Insecta Odonata

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No Wolbachia was detected in the gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TGGGnnGGnNnAATTGGTACGGCACTAAGAGTTTTAATTCGAATTGAATTGGGTCAACCCGGATCCCTAATTGGCGATGA TCAAATTTATAATGTGATTGTAACTGCACATGCATTTGTTATAATTTTCTTCATAGTAATACCTATTATAATTGGGGGTT TCGGAAACTGATTAGTACCCCTAATATTAGGAGCGCCAGATATGGCCTTCCCCCGATTGAATAACATAAGATTCTGACTT TTACCCCCCTCTTTCACATTACTTTTAGCAAGTAGAATGGTTGAAAGAGGGGCAGGAACAGGTTGAACTGTATACCCTCC TTTAGCTGGGGCAATTGCACATGCTGGAGCCTCAGTAGATTTAACCATTTTTTCTCTACATTTGGCTGGGGTATCATCAA TTCTAGGAGCGATTAATTTTATTACAACCGTAATCAATATGAAGTCACCAGGAATGAAGTTAGATCAGCTGCCATTATTT GTATGAGCAGTAGTAATTACTGCAGTATTGTTATTATTATCACTTCCTGTATTAGCTGGGGCTATTACAATGTTGTTAAC TGATCGAAATATTAATACATCCTTCTTTGATCCnGCAnGAGGGGGGGnnCCAATTCTTTACnnACACCTCTTCnnAnTTT T
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Odonata was found to be negative for Wolbachia.
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