Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Slow, does not try to climb up cup when captured, small sized (about .5 cm in length from start of head to end of abdomen) |
| Putative identification | |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Gel Images: Lane 6 for both (checking for Wolbachia presence and Arthropod extraction verification) |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | Confidence level is based on the fact that there were good extraction and results for the arthropod. The wolbachia was unsure so it was not sent to be sequenced. Additional Notes: After running the BLAST, the arthropod was determined to be an Asian needle ant (Brachyponera chinensis) |
| Wolbachia 16S sequence |
n/a
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid