Mealworm (Tenebrio molitor)

April 9, 2025

Sample information
Picture
Photos by: Vidya V.
Location
Collection date 11/13/2024
Captive / Cultivated? Captive / Cultivated
Group Walton High School
Observations

Tends to hide from the light, crawls fast towards darker areas trying to escape light. Found at Petco for classroom pet food. No location written on website purchased to where the mealworms were cultivated.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Gel Images: Lane 7 for both

(checking for Wolbachia presence and Arthropod extraction verification)

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

Confidence level is based on the fact that there were good extraction and results for the arthropod. The wolbachia was unsure so it was not sent to be sequenced.
Wolbachia 16S sequence
n/a
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
Summary
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