Black Carpenter Ant

November 18, 2024

Sample information
Picture
Photos by: Vidya V
Location
Collection date 11/13/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations
  • Was located under dirt and actively moving around, occasionally going under the leaves and dirt. Has a body bigger than the average black carpenter ant with a large abdomen.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Gel Image: Lane 9 and 10

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level
  • The controls in the gel had a faint band which could suggest the possibility of the controls not working, so the bands may have been an outlier that doesn’t necessarily correspond to the DNA ladder since the ladder didn’t properly show up either.
Wolbachia 16S sequence Download AB1
>Lab5Wolbachia A new nucleotide sequence entered manually GTTGCAGCAATGGTAGACTCAACGGTAGCAATAACTGCAGGACCTAGAGGAAAAACAGTAGGGATTAATAAGCCCTATGG AGCACCAGAAATTACAAAAGATGGTTATAAGGTGATGAAGGGTATCAAGCCTGAAAAACCATTAAACGCTGCGATAGCAA GCATCTTTGCACAGAGTTGTTCTCAATGTAACGATAAAGTTGGTGATGGTACAACAACGTGCTCAATACTAACTAGCAAC ATGATAATGGAAGCTTCAAAATCAATTGCTGCTGGAAACGATCGTGTTGGTATTAAAAACGGAATACAGAAGGCAAAAGA TGTAATATTAAAGGAAATTGCGTCAATGTCTCGTACAATTTCTCTAGAGAAAATAGACGAAGTGGCACAAGTTGCAATAA TCTCTGCAAATGGTGATAAGGATATAGGTAACAGTATCGCTGATTCCGTGAAAAAAGTTGGAAAAGAGGGTGTAATAACT GTTGAAGAGAGTAAAGGTTCAAAAGAGTTAGAAGTTGAGCTGACTACTGGCATGCAATTTGATCGCGGTTATCTCTCTCC GTATTTTATTACAAATAATGAAAAAATGATCGTGGAGCTTGATAATCCTTATCTATTAATTACAGAGAAAAAATTAAATA TTATTCAACCTTTACTTCCTATTCTTGAAGCTATTGTTAAATCTGGTAAACCTTTGGTTATTATTGCAGAGGATATCGAA GGTGAAGCATTAAGCACTTTAGTTATCAATAAATTGCGTGGTGGTTTAAAAGTTGCTGCAGTAAAAGCTCCAGGTTTTGG TGACAGAAGAAAGGAGATGCTCGAAGACATAGCAACTTTAACTGGTGCTAAGTACGTCATAAAAGATGAACTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download AB1
TATTTGATTTTTGGAGCTTGAGCTGCTATAGTAGGTACAGCAATAAGAGTATTAATCCGTATAGAATTGGGTCAAACTGGAAATTTTATAGGTGATGATCATTTATATAATGTAATTGTAACTGCTCATGCTTTTGTAATGATTTTTTTTATAGTAATGCCTATTATAATTGGAGGTTTTGGTAATTGATTAGTTCCTTTAATGTTGGGGGCTCCAGATATAGCTTTTCCCCGAATAAATAATTTGAGTTTTTGATTATTACCCCCTTCTCTAATATTATTATTTATTTCTTCCATAGCAGAAATAGGGGTTGGAGCTGGATGAACAGTATATCCACCTTTGGCATCCATTGTAGGTCATAATGGAAGCTCAGTAGATTTTGCCATTTTTTCTTTACATTTGGCGGGTGCTTCATCTATTATAGGGGCTATTAATTTTATTTCTACTGTTATTAATATACGGTCAATTGGAATATTCTTAGACAAGGTACCTTTGTTTGTATGATCTGTAGTTATTACGGCCGTATTATTACTTCTTTCATTACCGGTATTGGCTGGTGCTATTACTATATTATTGACTGATCGTAATTTTAATACTTCTTTTTTTGATCCTGCAGGAGGGGGGGACCCTATTTTATTTCAACATTTGTTTTGATTTTTGTCCCCCTNNAAAAAATTTAAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be postive for Wolbachia.
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