Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/14/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations |
The moth fly was mainly stationary and resting on a wall and when captured, flew around a while before resting again on wall of container again. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Psychodidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Gel image: Lane 9 and Lane 10 |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Our DNA ladder wasn’t clear when migrating so the bands may not correlate to the Wolbachia gene, however there was no band shown for the moth fly well so there is some assurance that the moth fly would not have had the wolbachia gene if the ladder did work. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Psychodidae was found to be negative for Wolbachia. |
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