Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/14/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Brownish in color, with a tint of red on the head. The abdomen has some black as well. |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Lasius |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Lane 11 |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Control groups did not group, positive did not show up and the negative showed up. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lasius was found to be negative for Wolbachia. |


Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid