Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Spider found in a web about 4 feet off the ground. First attempted capture failed and spider moved away, but second attempt succeeded in capture. Spider formed a web in container before it was killed. Has eight furry legs and a relatively small body. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Agelenidae Barronopsis Barronopsis texana |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | The controls did not work ver effectively, however there is a clear band at 438bp so it appears to contain Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Barronopsis texana was found to be postive for Wolbachia. |
Oncopeltus fasciatus
Formica Pallidefulva
Hylephila Phyleus
Thomisus onustus
Halictus Ligatus