Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Found in moist soil near a creek in a forest. |
| Putative identification | Arthropoda Diplopoda Julida Parajulidae Ptyoiulus Ptyoiulus impressus |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | Both of the samples are in lane 3 of the gels. |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
GAACAGGTTGAACAGTCTATCCCCCCTTAGCTAGTAACGTATCACACTCCGGAGCTTCAGTAGACATAGCAATTTTTTCACTTCATCTAGCAGGGGCCTCCTCAATTCTAGGAGCCATTAATTTTATTACCACAATTATTAATATACGAAGAGCAGGTATGTTGTTTGAACAAATACCCTTATTTGTGTGGGCAGTAAAAATTACAGCCATTTTACTTCTTTTATCTCTCCCCGTTTTAGCTGGTGCTATTACTATGCTCTTAACAGATCGGAACTTTAACACTTCTTTTTTCGACCCTGCAGGGGGAGGAGACC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid