Black carpenter ant

November 20, 2024

Sample information
Picture
Photos by: Sara J.
Location
Collection date 11/12/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

In moist soil, found under a bed of leaves in a foresty area.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Sample is in lane 2 in both of the gels

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There was no bar at the 4
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Camponotus pennsylvanicus was found to be negative for Wolbachia.
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