Portland State University – Pieris rapae

November 25, 2024

Sample information
Picture
Photos by: Jesse Porter
Location
Collection date 10/15/2024
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Insecta Lepidoptera Pieridae Pieris Pieris rapae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Gel B, wells 2-4. Well 2 is positive for the insect’s DNA, well 3 is a positive control for Wolbachia DNA, and well 4 is negative for Wolbachia in the insect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Pieris rapae was found to be negative for Wolbachia.
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