Portland State University – Formicidae

November 25, 2024

Sample information
Picture
Photos by: Ahmad Nelson
Location
Collection date 10/15/2024
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Gel B, Wells 14-16. Well 14 is positive for arthropod DNA, well 15 is a positive control for Wolbachia, and well 16 is negative for Wolbachia in the insect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Formicidae was found to be negative for Wolbachia.
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