Portland State University – Tapinoma sessile

November 25, 2024

Sample information
Picture
Photos by: Jaskaran Singh
Location
Collection date 10/16/2024
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Insecta Hymenoptera Formicidae Tapinoma Tapinoma sessile

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Gel B, wells 11-13. Well 11 is positive for insect DNA, well 12 is positive for Wolbachia positive control, well 13 is negative for the insect’s Wolbachia test.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Tapinoma sessile was found to be negative for Wolbachia.
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