Sal’s Wasp

November 26, 2024

Sample information
Picture
Photos by: Sal Ahmed
Location
Collection date 09/24/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

It was a dry and hot day. Collected from the marshy area by the garage.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit tweezers
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer Other
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Wolbachia band was displayed on the gel electrophoresis.
Wolbachia 16S sequence
CACGGNGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GCTAGAACAGGTAAAGATAATAAAAGTAAAATTGCAGTAATTAAAACTGATCATGTAAATATAGGTAAAAAATTTAATGA ATGAGTTTTAATGTGTATATTTAAGATTGTTACAATGAAATTAATAGCTCCTATGATTGATGAAATTCCTGCAATGTGAA GAGAGAAAATAGCAAAATCAACTGATGATGAATTGTGACCTATATTAGATGATAAAGGTGGATATAAAGTTCATCCAGTT CCTACTCCTATATCATTAATATTTCTAATAATTAATAATAATAATGATGGTGGTAATAATCAAAATCTTATATTATTTAT TCGTGGGAATGCTATATCTGGGGCACCTAGTATTAAGGGAATTAATCAATTTCCAAATCCTCCAATTATAAATGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be postive for Wolbachia.
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