Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/31/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | North Penn-Mansfield JSHS |
| Observations | Location – Steep streamside dirt Notes – Sprinkling rain, multiple of same species observed |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Lasius |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All control lanes came back as expected, the test lane returned a single defined band in the 1000bp-500bp range, parallel to the control negative arthropod. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lasius was found to be negative for Wolbachia. |
Bombus impatients (Eastern Bumblebee)
Agelenopis
My Data
Common Eastern Bumble Bee (Bombus impatiens)
American Bird