Formica Glacialis

March 31, 2025

Sample information
Picture
Photos by: Isaak S
Location
Collection date 09/28/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

On stairs outdoors, surrounding food left outside, with others of its own, fall, 50 degrees F
Putative identification Arthropoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Lane 4 is the observed arthropod.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Clear two strains in gel electrophoresis. Wolbachia DNA found by matching in BLAST.
Wolbachia 16S sequence Download FASTA    Download AB1
GCTCGTGTCGTGAGAnGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CTTCTATAAGnATAATTATTCGTTTAGAATTAGGTTCGTCTAATTCATTAATCAATAATGATCAAATTTATAATTCTTTAGTAACTAATCACGCTTTTATTATAATTTTTTTCATAGTAATACCATTTATAATTGGTGGATTTGGAAATTTTCTAATTCCTTTAATACTAGGATCACCAGACATAGCATACCCTCGTATAAATAATATAAGATTTTGACTCTTACCTCCTTCAATTACTCTTTTACTTTTAAGAAATTTTATTAATGATGGAACAGGAACAGGATGGACTATTTATCCTCCCTTATCCTCTAATATTTTTCATAATGGTCCTTCTGTAGACTTAACAATTTTTTCTCTTCATATTGCAnGnATATCCTCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTCGTATGATCAATTTTAATTACAGCTATCTTACTTCTATTATCCTTACCTGTATTGGCCGGAGCTATTACTATACTATTAACTGATCGAAATTTAAATACTTCATTCTTTGATCCTTCGGGGGGAGGTGATCCAATTCTATATCAACATTTA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be postive for Wolbachia.
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