Aedes Trivittatus

December 2, 2024

Sample information
Picture
Photos by: Ella S., Ashley C.
Location
Collection date 09/19/2024
Captive / Cultivated? Wild-caught
Group Gettysburg College
Observations

CO1 sequence –

ANTCATAAAGATATNGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTCTTATCTCTCCCTGTATTAGCCGGAGCTATTACAATATTATTAACTGACCGAAATCTAAATACATCTTTCTTTGACCCAATTGGTGGAGGAGATCCAATTCTATATCAACATTTATTCTGATTTTT
Putative identification Arthropoda Insecta Diptera Culicidae Aedes Aedes trivittatus

Methods
Extraction kit Qiagen Blood and Tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The area in which we obtained it from did not have the type of mosquito that would be wolbachia positive. No bands were at the right size, meaning that our wolbachia was wolbachia negative.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Aedes trivittatus was found to be negative for Wolbachia.
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