Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/04/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2024 students (sweep net) |
| Putative identification | Arthropoda Hexapoda Insecta Odonata |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | Possible contamination, 100% DNA and Wolbachia results received for control arthropod (fruit fly). |
| Wolbachia 16S sequence | Download FASTA
|
| Arthropod COI sequence | Download FASTA
|
| Summary | |
Water Boatman