Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/04/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | collected by Fall 2024 students |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Based on the results of the gel it appeared that no Wolbachia was present. My confidence level is low because there could have been an error during PCR, such as unsuccessful amplification, non-specific binding or degraded primers, which could have led to false/negative results. |
| Wolbachia 16S sequence | Download FASTA
|
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be negative for Wolbachia. |
Water Boatman