Sample information
Picture	Photos by: Kate L
Location
Collection date	09/04/2024
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	Collected by Fall 2024 students
Putative identification	Arthropoda Hexapoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level	Thick gel band with over 99% BLAST match
Wolbachia 16S sequence
Arthropod COI sequence	Download FASTA    Download AB1
Summary	The Harmonia axyridis was found to be negative for Wolbachia.