Asian Lady Beetle (F.H.)

December 6, 2024

Sample information
Picture
Photos by: Fatima H.
Location
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

It was found near white flowers in the grassland area near the parking  lot

it’s red and spotted

looks like a ladybug
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit pcr
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

It only showed one band in gel electrophoresis indicating no Wolbachia and the presence of DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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