Aedes albopictus

December 8, 2024

Sample information
Picture
Photos by: Jess and Kristen
Location
Collection date 09/23/2024
Captive / Cultivated? Wild-caught
Group Gettysburg College
Observations

Suburban neighborhood
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Aedes Aedes albopictus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Lane 10

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Positive control positive, negative control negative
Wolbachia 16S sequence
Arthropod COI sequence
ANTAGGGTCTCCCCCTCCAATTGGATCAAAAAAAGATGTATTTAAATTTCGGTCTTTGATCTAATATAGTCGGAACTTCGCTTAATACGGGTAGAGAAAGAAGTAATAAAAGAGTTTTAATTCGTATTGAACTTAGACATCAAGGTAGTCGATCAAGAGTAATACCAGCTGATCGTATATTAATTGGAAATGTAATAAAATTTATTGCTCCTAAAATAGATGAGATTCCCGCTAAATGTAAAGAAAAAATTGCTAAATCAACTGAAGCCCCAGCATGAGCTGTTCCAGAAGAAAGAGGAGGATAAACCGTTCACCTTGTTACTGCTCATGCTTTTATTATAGAACTAGAAAGCAGCAGTGTTAAAGAGGGGGGTAATATTCAAAAACTTATTTTTTTTATTCGAGGAAAAGCTATATCAGGGGCTCCTAGTAATAAGGGTACTAGTCAGTTTCCAAATCCTCCAATTATGATAGGTATTACTATAAAAAAAATCATAATTGGAGCATGAGCAGTAACAATTACATTGGAAATCTGACTAGTACCCTTAAATATACTAGGATGTCTAAGTTCAATACGAATTAAAGCCCCTGATATAGTTCCGACTTTTCCAGATCGAATACCGAAAATAATATATAGTTTTTGAGTATTAGATCTGGAATAGTCGGAACTTCACTAAGAGTTTTAATTCGTATTGAACTTAGACATCCTGGTATATTTATTGGAAATGATCAAATTTATAATGTAATTGTTACTGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATCATAATTGGAGGATTTGGAAACTGACTAGTACCCTTAATACTAGGAGCCCCTGATATAGCTTTTCCTCGAATAAATAATATAAGTTTTTGAATATTACCCCCCTCTTTAACACTGCTGCTTTCTAGTTCTATAGTAGAAAACGGAGCTGGAACAGGGTGAACGGTTTATCCTCCTCTTTCTTCTGGAACAGCTCATGCTGGGGCTTCAGTTGATTTAGCAATTTTTTCTTTACATTTAGCGGGAATCTCATCTATTTTAGGAGCAGTAAATTTTATTACAACTGTAATTAATATACGATCAGCTGGTATTACTCTTGATCGACTACCTTTATTTGTGTGATCAGTAGTAATTACAGCTATTTTATTACTTCTTTCTCTACCCGTATTAGCCGGAGCTATTACTATATTATTAACAGACCGAAATTTAAATACATCTTTTTTTGATCCAATTGGAGGGGGAGACCCTATTTTATATCAACATTTATTTTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Aedes albopictus was found to be postive for Wolbachia.
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