Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/04/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | It was caught in the grass with a killing jar. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Vespidae Vespula Vespula maculifrons |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | There was no presence of Wolbachia in my arthropod, but rather it was present in my partner’s. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
>74-C_AG1_Co1R.scf (612 bp) CGAAAAAAGATGTGTTTAAGTTCCGATCTGTTAAAAGTATAGTAATNGCTCCTGCAAGAACTNGTAGTGATAATAGAAGA AGAATTGCTGTAATTAAAATTGATCATGTGAATAAAGGAAGAAAATTTAATGAATGTGTTTTTGTGTGTATATTTAAAAT AGTAACGATAAAATTAATTGATCCTATAATTGATGAAATTCCAGCAATATGAATTGAAAAAATTCCTAAATCTACAGATG GAGAATCATGACCAACAATAGAAGATAAAGGAGGGTATAAAGTTCATCCTGTTCCTACCCCTGTTCCAATAAAATTTCTT AAAATTAGTAAAAATAATGAAGGAGGGAGTAATCAAAATCTTATATTATTTATTCGAGGAAATGCTATATCAGGTACACC TAGTATTAAAGGGATTAATCAGTTTCCAAATCCTCCTACTAAAAAAGGTATAACTATAAAGAAAATTATAATAAAGGCAT GAGCTGTAATAATTGNATTATAAATTTGATCNTTATTAATTAANNNTNCNGGANANCTTAATTCTAAGCNAANAANTATT CTNATNGAAGCTCCTAAAGTCCCTGCCCATNAAGCAAAAANAAAATANAATN
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Vespula maculifrons was found to be negative for Wolbachia. |
Water Boatman