Sample information
Picture	Photos by: Aliyah Jones
Location
Collection date	09/04/2024
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	Collected by me was collected using a sweeping net on the concrete sidewalk.
Putative identification	Arthropoda Hexapoda Insecta Orthoptera Acrididae Melanoplus Melanoplus femurrubrum
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Partial abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Melanoplus femurrubrum was found to be negative for Wolbachia.