Formica Glacialis

March 31, 2025

Sample information
Picture
Photos by: IS
Location
Collection date 09/28/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

on food plate outside 50 degrees F.
Putative identification Arthropoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

observe lane 3

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Clear Double strands in gel. Wolbachia DNA found matched in Blast
Wolbachia 16S sequence Download FASTA    Download AB1
NNNNGCTCGTGTCGTGAGAnGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
NNNNNNNNNNNNNNNNGCTATTTGAGCAGGAATAATTGGATCTTCTATAAGTATAATTATTCGTTTAGAATTAGGTTCGTCTAATTCATTAATCAATAATGATCAAATTTATAATTCTTTAGTAACTAATCACGCTTTTATTATAATTTTTTTCATAGTAATACCATTTATAATTGGTGGATTTGGAAATTTTCTAATTCCTTTAATACTAGGATCACCAGACATAGCATACCCTCGTATAAATAATATAAGATTTTGACTCTTACCTCCTTCAATTACTCTTTTACTTTTAAGAAATTTTATTAATGATGGAACAGGAACAGGATGGACTATTTATCCTCCCTTATCCTCTAATATTTTTCATAATGGTCCTTCTGTAGACTTAACAATTTTTTCTCTTCATATTGCAGGTATATCCTCAATTTTAGGAGCAATTAATTTTATTTCAACAATTCTTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTCGTATGATCAATTTTAATTACAGCTATCTTACTTCTATTATCCTTACCTGTATTGGCCGGAGCTATTACTATACTATTAACTGATCGAAATTTAAATACTTCATTCTTTGATCCTTCGGGGGGAGGTGATCCAATTCTATATCAACATTTATTTTGATTTTTTGGTCACCCNNNNAAGTTTAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be postive for Wolbachia.
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