Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/04/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations |
Sweep net while on a bush |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes |
|
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Due to the thick band and my BLAST results |
| Wolbachia 16S sequence |
GGCTGTCGT CAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC CTCATCCTTAGTTGCTATCAGGT AATGCTGAGTACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGAT GTCAAGTCATCATGGCCTTTATG GAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAGGTGCGCAAGC CTAAGCTAATCCCTAAAAGACAT CTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATC GTGGATCAGCATGCCACGGTGAA TACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
N/A
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
Collected by Fall 2024 students
Water Boatman