Sample information
Picture	Photos by: Evan B
Location	No map location data available.
Collection date	11/07/2025
Captive / Cultivated?	Wild-caught
Group	John Bapst Memorial High School
Observations
Putative identification	Arthropoda Insecta Coleoptera
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TBE
DNA stain	Other
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Coleoptera was found to be negative for Wolbachia.