Firefly

Sample information

Picture
Photos by: Evan B
Location No map location data available.
Collection date 11/07/2025
Captive / Cultivated? Wild-caught
Group John Bapst Memorial High School
Observations
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain Other
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level Low
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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