Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/07/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | John Bapst Memorial High School |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We have high confidence in these results because all our negative controls worked, our positive controls all tested positive. There seems to be no contamination. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Strawberry Shortcake
Grub Ahub Dub
Japanese Lady Bug
house fly