Unknown Black Fly

March 29, 2021

Sample information
Picture
Photos by: Isabella R.
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Bordenstein Lab
Observations

found on aster in a flowerbed, NE location in backyard behind suburban home

2mm long, all black, two wings, delicate legs
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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