Unknown Yellow Fly

Sample information

Picture
Photos by: Isabella R.
Location
Collection date 10/04/2020
Captive / Cultivated? Wild-caught
Group Bordenstein Lab
Observations

caught in suburban back yard on an impatient flower; caught at 2:15 p.m.; 73 degrees F

 

~1cm long, looks like a fly with black legs and thorax, and a yellow abdomen, two wings, large brown eyes

Putative identification Arthropoda Hexapoda Insecta Diptera

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be postive for Wolbachia.
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