Sample information
Picture	Photos by: Ahmed A.
Location
Collection date	09/26/2024
Captive / Cultivated?	Wild-caught
Group	Woburn Memorial High School
Observations
Putative identification	Arthropoda Hexapoda Insecta Orthoptera Acrididae Dissosteira Dissosteira carolina
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Gonads dissected
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	Controls were correct, protocol was followed well.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Dissosteira carolina was found to be negative for Wolbachia.